Inflammation Methods and Protocols [Paperback]

This volume presents a broad selection of cutting-edge methods and tools that will enable the reader to investigate the multi-faceted manifestations of inflammation. Inflammation: Methods and Protocols is divided into four sections: the first three sections describe protocols investigating immune-mediated inflammatory disease models affecting barrier organs to the environment; the skin, the lung, and the intestinal and oral mucosa. The fourth section illustrates inflammatory disease models of the brain, joints, and vasculature. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Inflammation: Methods and Protocols aims to inspire the experienced investigator and the young experimenter alike to disentangle the fascinating process of inflammation.

 Reproducibility Issues: Avoiding Pitfalls in Animal Inflammation Models.- Hapten-specific T cell-mediated Skin Inflammation: Flow Cytometry Analysis of Mouse Skin Inflammatory Infiltrate.- Monitoring Skin Dendritic Cells in Steady State and Inflammation by Immunofluorescence Microscopy and Flow Cytometry.- Visualization of the T Cell Response in Contact Hypersensitivity.- Ultraviolet Radiation-induced Immunosuppression  Induction of Regulatory T cells.- Surgical Denervation in the Imiquimod-Induced Psoriasiform Mouse Model.- Xenotransplantation Model of Psoriasis.- A Mouse Model for Atopic Dermatitis using Topical Application of Vitamin D3 or of its Analog MC9039 Particle Bombardment of Ex-vivo Skin to Deliver DNA and Express Proteins.- Mouse Models of Asthma.- Subcutaneous and Sublingual Immunotherapy in a MlS