This volume presents standard approaches and the most recent technical advances used to study innate immune activation. Chapters detail the assessment of macrophage activation, measuring innate immune responses to bacterial viability, quantification of secreted proteins, reporter systems, protocols examining specific innate immune activation by TLRs, RLRs, cGAS, and inflammasomes. Written in the highly successful?Methods in Molecular Biology?series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical,?
Innate Immune Activation: Methods and Protocols?aims to be a useful and informative reference tool for further study into this vital field.
1????????? Emerging Concepts in Innate Immunity
Karin Pelka and Dominic De Nardo
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2????????? Bioinformatic Assessment of Macrophage Activation by the Innate Immune System
Thomas Ulas, Joachim L. Schultze, and Marc Beyer
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3????????? Generation of Genetic Knockouts in Myeloid Cell Lines using a Lentiviral CRISPR/Cas9 System
Paul J. Baker and Seth L. Masters
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4????????? Modeling Primary Human Monocytes with the Trans-differentiation Cell Line BLaER1
Moritz M. Gaidt, Francesca Rapino, Thomas Graf, and Veit Hornung
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5????????? Measurement of NF-?B Activation in TLR-activated Macrophages
Orna Ernst, Sharat J. Vayttaden, and Iain D.C. Fraser
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6????????? Biochemical Isolation of the Myddosome from Murine Macrophages
Yunhao Tan and Jonathan C. Kaganl#Ý
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