Integrin Protocols [Paperback]

Part 1 Working with Integrins. Integrin-Binding Peptides Derived from Phage Display Libraries, Erkki Koivunen, Bradley H. Restel, Daniel Rajotte, Johanna Lahdenranta, Martin Hagedorn, Wadih Arap, and Renata Pasqualini. Production of Rat Monoclonal Antibodies Specific for Mouse Integrins, Marc Delcommenne and Charles H. Streuli. Biochemical Analysis of Integrin-Mediated Shc Signaling, Kishore K. Wary, Michael Dans, Agnese Mariotti, and Filippo G. Giancotti. Assays for Kinase Activity, Maja H. Oktay, Agnese Mariotti, and Filippo G. Giancotti. Immunoblotting of Integrins, Maximilian Schneller, Wadih Arap, and Renata Pasqualini. Application of Flow Cytometry in the Analysis and Sterile Sorting of Cell Populations Based on Integrin Expression, Edward C. Rosfjord and Robert B. Dickson. Expression of Integrin Transcripts in Human Cancer Cells, Wadih Arap and H.-J. Su Huang. Part 2 Functional Analysis of Integrins. Functional Analysis of the b2 Integrins, Rui Li and M. Amin Arnaout. Expression of Heterologous Integrin Genes, Craig A. Steger, Mara Fornaro, Amy S. Woodard, Duo-Qi Zheng, and Lucia R. Languino. Preparing a Polyclonal Antibody to Mouse b1 Integrin with Function-Blocking Activity, Gwynneth M. Edwards and Charles H. Streuli. Integrin Gene Targeting, Jan F. Talts, Cord Brakebusch, and Reinhard F?ssler. Determination of Positive and Negative Regulatory Elements, Emilio Hirsch, Piero Cervella, Lorenzo Silengo, and Fiorella Altruda. Part 3 Integrin Function at the Cellular Level. A Cell-Based Adhesion Assay for the Characterization of Integrin avb3 Antagonists, James M. Wu, Mary P. Rosser, Anthony R. Howlett, and Richard I. Feldman. Integrins in Cell Migration, Klaus von der Mark, Stephan Sch?ber, and Simon L. Goodman. Models for Studying Cellular Invasion of Basement Membranes, John T. Price and Erik W. Thompson. Methods for Studying Anoikis, Steven M. Frisch. Use of the 10-Day-Old Chick Embryo Model for Studying Angiogenesis, Peter C. Brooks, Anthony M. P.Montgomeryl#.