Intermediate Filament Associated Proteins [Hardcover]

Intermediate Filament Associated Proteins, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in intermediate filament associated proteins and contains sections on such topics as lamin-associated proteins, intermediate filament-associated proteins and plakin, and other cytoskeletal cross-linkers.

• Continues the legacy of this premier serial with quality chapters authored by leaders in the field
• Covers research methods in intermediate filament associated proteins and contains sections on such topics as lamin-associated proteins, intermediate filament-associated proteins and plakin, and other cytoskeletal cross-linkers

Part I: Identification and Biochemical Analysis of Lamin-Associated Proteins
1.?BioID Identification of Lamin-Associated Proteins
Aaron A. Mehus, Ruthellen H. Anderson and Kyle J. Roux
2.?Proximity-Dependent Biotin Identification (BioID) in Dictyostelium Amoebae
Petros Batsios, Irene Meyer and Ralph Gr?f
3.?Purification and Structural Analysis of LEM-Domain Proteins
Isaline Herrada, Benjamin Bourgeois, Camille Samson, Brigitte Buendia, Howard J Worman and Sophie Zinn-Justin
4.?Purification and Structural Analysis of SUN and KASH Domain Proteins
F. Esra Demircioglu, Victor E. Cruz and Thomas U. Schwartz
5.?Purification of Lamins and Soluble Fragments of NETs
Alexandr A. Makarov, Andrea Rizzotto, Peter Meinke and Eric C. Schirmer
6.?Simple Separation of Functionally-Distinct Populations of Lamin-Binding Proteins
Jason M. Berk and Katherine L. Wilson
Part II: General Methods for Analysis of Intermediate Filament-Associated Proteins
7.?Development of a Novel Green Fluorescent Proln