Ion Channels Methods and Protocols [Hardcover]

The diverse applications in this volume range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, and for studying channel regulation.Internationally recognized biomedical scientists describe recent technological breakthroughs and demonstrate their use in successful experimental designs. The diverse applications range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, for studying channel regulation and physiological function, and for genetic screening and investigating channelopathies.Part I. Methods for Exogenous Expression of Ion Channels in CellsFunctional Reconstitution of the Human Epithelial Na+ Channel in a Mammalian Expression SystemAlexander Staruschenko, Rachell E. Booth, Oleh Pochynyuk, James D. Stockand, and Qiusheng TongOverexpression of Proteins in Neurons Using Replication-Deficient VirusRichard M. Ahlquist and Jane M. SullivanExogenous Expression of Proteins in Neurons Using the Biolistic Particle Delivery SystemNikita Gamper and Mark S. ShapiroPart II. Methods for Studying Channel Structure-FunctionTertiary and Quaternary Structure Formation of Voltage-Gated Potassium ChannelsJohn M. Robinson, Andrey Kosolapov, and Carol DeutschBiophysical Approach to Determine the Subunit Stoichiometry of the Epithelial Sodium Channel Using the Xenopus laevis Oocyte Expression SystemFarhad Kosari, Shaohu Sheng, and Thomas R. KleymanSpectroscopy-Based Quantitative Fl“ê