Isolation of Plant Organelles and Structures Methods and Protocols [Paperback]

This book brings together the major techniques used in the isolation or enrichment of individual populations of organelles and other subcellular structures from plants with the goal that, by being able to isolate subcellular structures, the research and understanding of various facets of compartmentalized function in plant cells can be advanced. Written for the highly successful Methods in Molecular Biology series, expert contributors provide chapters that contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

1. The Isolation of Plant Organelles and Structures in the Post-Genomics Era

            A. Harvey Millar and Nicolas L. Taylor

 

2. Approaches to Characterize Organelle, Compartment, or Structure Purity

            Stefanie J. Mueller, Sebastian N.W. Hoernstein, and Ralf Reski

 

Part I: Isolation of Organelles and Organelle Compartments

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3. Isolation of Nucleil³ê