Multiple Sclerosis Methods and Protocols [Hardcover]

This detailed volume covers molecular biology, cellular biology, biomarkers, imaging, and neuropathological methods and techniques to explore multiple sclerosis (MS), with a special emphasis on disease models. With so much effort needed to elucidate basic disease mechanisms, to clone disease relevant genes, to define novel biomarkers, and to discover novel and improved therapeutic and curative treatments, this book serves to aid researchers in accomplishing these enormous goals.? Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and easy to use, Multiple Sclerosis: Methods and Protocols will empower the reader to perform novel research regarding pathophysiology and treatment for MS.

Part I: Molecular Biology Techniques

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1. Positional Gene Cloning in Experimental Populations

??????????? Maja Jagodic and Pernilla Stridh

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2. Generation of Transgenic Rats Using Lentiviral Vectors

??????????? Holger M. Reichardt and Henrike J. Fischer

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3. DNA Vaccination Techniques

??????????? Nicol?s Fissolo, Xavier Montalban, and Manuel Comabella

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Part II: Cellular Techniques

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4. Peripheral Blood Mononuclear Cells: Isolation, Freezing, Thawing, and Culture

??????????? Christine Riedhammer, Dagmar Halbritter, and Robert Weissert

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5. ELISPOT Techniques

??????????? Niannian Ji and Thomas G. Forsthuber

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6. Isolation of lÃ4