Yeast Functional Genomics Methods and Protocols [Hardcover]

This volume provides a?collection of protocols for the study of DNA-DNA contact maps, replication profiles, transcription rates, RNA secondary structures, protein-RNA interactions, ribosome profiling and quantitative proteomes and metabolomes. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Yeast Functional Genomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.

1. Using RNA-seq for Analysis of Differential Gene Expression in Fungal Species

Can Wang, Markus S. Schr?der, Stephen Hammel, and Geraldine Butler

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2. Enhancing Structural Annotation of Yeast Genomes with RNA-Seq Data

Hugo Devillers, Nicolas Morin, and C?cile Neuv?glise

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3. Pathogen Gene Expression Profiling During Infection Using a Nanostring Ncounter Platform

Wenjie Xu, Norma V. Solis, Scott G. Filler, and Aaron P. Mitchell

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4. Comparative Transcriptomics in Yeasts

Dawn A. Thompson

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5. Mapping the Transcriptome-Wide Landscape of RBP Binding sites using gPAR-CLIP-seq: Experimental Procedures

Ting Ha and John K. Kim

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6. Mapping the Transcriptome-wide Landscape of RBP Binding Sites using gPAR-CLIP-seq: Bioinformatic Analysis

Mallory A. Freeberg and John K. Kim

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7. Translation Analysis at the Genome Scale by Ribosome Profiling<l³!