Ancient DNA Typing Methods, Strategies and Applications [Paperback]

This is the definitive source of information on techniques for the identification and sequencing of old DNA (pieces) and their use in biological and medical research and application. Application of aDNA techniques are useful tools for investigations reaching from evolutionary studies to law enforcement approaches. What brings them together is the interest in specific methods of handling aDNA, i.e. elaborated PCR and sequencing techniques and the interpretation of the results. This books serves as an ideal guideline for it demonstrates how problem-solving strategies can be applied in various areas.Ancient DNA Typing is a comprehensive manual about the analysis of ancient and degraded DNA. It is an equally useful textbook and working tool for various disciplines such as: Anthropology, Archaeology, Forensic Medicine, Conservation Biology, Environmental Sciences and Food Engineering.
The reader benefits from a clear division of strategies, methods and applications with many interesting examples of the analysis of historic skeletal and cultural remains. The book contains a detailed protocol chapter which serves as a lab manual with the most important methods for DNA extraction, PCR and electrophoretic analysis of degraded samples. The appendix helps to set up a PCR lab specializing in ancient DNA research and answers the most frequent questions concerning technical problems in investigations of degraded DNA.1 Introduction.- 2 DNA markers.- 3 aDNA extraction.- 4 PCR.- 5 Analysis of PCR products.- 6 Authenticity of results.- 7 Applications.- 8 Protocols.- I Laboratory equipment.- I.1 Pre-PCR laboratory equipment.- I.2 PCR laboratory.- I.3 Post-PCR laboratory.- I.4 Photographic documentation.- II PCR trouble shooting.- III Internet sites of interest.

From the reviews:

This is a book that does what it says on the cover. Written by one of the leaders in the aDNA field, it covers both the basic techniquelƒ&