Antibiotic Resistance Protocols [Hardcover]

This third edition provides a wide range of different technologies, ranging from conventional growth basic techniques, application of molecular biology, development of resistance mutations, and diagnosis and monitoring treatment response.? New and updated chapters cover techniques from the microscopic scale to whole animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Antibiotic Resistance Protocols, Third Edition aims to ensure successful results in the further study of this vital field.

1.? Methods for Measuring the Production of Quorum Sensing Signal Molecules

Manuel Alcalde-Rico and Jos? Luis Mart?nez

2.? Construction and Use of Staphylococcus aureus Strains to Study Within-Host Infection Dynamics?

Gareth McVicker, Tomasz K. Prajsnar, and Simon J. Foster

3.? Method for Detecting and Studying Genome-wide Mutations in Single Living Cells in Real-time?

Marina Elez, Lydia Robert, and Ivan Matic

4.? Detecting Phenotypically Resistant Mycobacterium tuberculosis using Wavelength Modulated Raman Spectroscopy

Vincent O. Baron, Mingzhou Chen, Simon O. Clark, Ann Williams, and Stephen H. Gillespie

5. A Flow Cytometry Method for Assessing M. tuberculosis Responses to Antibiotics?? Charlotte L. Hendon-Dunn, Stephen R. Thomas, Stephen C. Taylor, and Joanna Bacon

6.? Application of Continuous Culture for Assessing Antibiotic Activity against Mycobacterium tuberculosilóC