Capillary Electrophoresis of Biomolecules Methods and Protocols [Paperback]

Capillary electrophoresis (CE) is a relatively new separation technique suitable for handling small amounts of sample very important in bioanalytical research and in various clinical, diagnostic, genetic, and forensic applications. In Capillary Electrophoresis of Biomolecules: Methods and Protocols, expert researchers in the field provide key techniques to investigate CE focusing on simple and complex carbohydrates (polysaccharides), aminoacids, peptides and proteins, enzymes, and nucleic acids. Along with practical procedures, reviews discussing CE applications related to bio(macro)molecules are also included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Capillary Electrophoresis of Biomolecules: Methods and Protocols provides the reader with the latest break throughs and improvements in CE and CE techniques applied to several classes of bio(macro)molecules.

This book details key techniques used to investigate Capillary electrophoresis (CE). It focuses on simple and complex carbohydrates (polysaccharides), aminoacids, peptides and proteins, enzymes, and nucleic acids.

1. Recent Advances in Capillary Electrophoresis-Based Proteomic Techniques for Biomarker Discovery

Chenchen Wang, Xueping Fang, and Cheng S. Lee

2. Capillary Electrophoresis-mass Spectrometry of Carbohydrates

^{Joseph Zaia}

^{3. Approaches to Enhancing the Sensitivyty of Carbohydrate Separations in Capillary Electrophoresis}

^{M.C. Breadmore}