Cell Biology Assays Essential Methods [Paperback]

This text provides comprehensive protocols essential methods across cell biology. The techniques in this text are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls while enabling researchers at all stages to embark on basic problems using a variety of technologies and model systems.

• Provides researchers with solutions in lab environments
• Features an array of essential methods, including endocytic pathways, membranes, mitochondria, and in vitro motility
• Information on a plethora of technologies needed to tackle complex problems

1. Permeabilized Epithelial Cells to Study Exocytic Membrane Transport
2. Studying Excit and Surface Delivery of Post-Golgi Transport Intermediates Using in vitro and Live-Cell Microscopy-Based Approaches
3. Assays Measuring Membrane Transport in the Endocytic Pathway
4. Microsome-Based Assay for Analysis of Endoplasmic Reticulum to Golgi Transport In Mammalian Cells
5. Cotranslational Translocation of Proteins into Microsomes Derived from the Rough Endoplasmic Reticulum of Mammalian Cells
6. Use of Permeabilsed Mast Cells to Analyze Regulated Exocytosis
7. Syringe Loading: A Method for Assessing Plasma Membrane Function as a Reflection of Mechanically Induced Cell Loading
8. Cell Surface Biotinylation and Other Techniques for Determination of Surface Polarity of Epithelial Monolayers
9. Protein Translocation into Mitochondria
10. Polarographic Assays of Mitochrondrial Functions
11. Analysis of Nuclear Protein Import and Export in Digitonin-Permeabilized Cells
12. Heterokaryons: An Assay for Nucleocytoplasmic Shuttling
13. DNA Replication-Dependent Chromatin Assembly SystemlÃJ