Cell Senescence Methods and Protocols [Hardcover]

?Cell senescence is the process whereby cells permanently lose the possibility to proliferate without undergoing cell death, and occurs in a plethora of distinct model organisms. In Cell Senescence: Methods and Protocols, expert researchers in the field detail the methods that are now commonly used to study cell senescence, in model organisms encompassing bacteria, fungi, worms, flies, zebrafish, and mammalian cells.?These techniques cover the study of all the morphological, biochemical and functional manifestations of senescence at the cellular level and include protocols for population analyses and high-throughput approaches in suitable model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

1. Cell Senescence as Both a Dynamic and a Static Phenotype

Andrew RJ Young, Masako Narita, and Masahi Narita

2. Senescence Regulation by mTOR

Vjekoslav Dulic

3. Senescence Regulation by the p53 Protein Family

Yingjuan Qian and Xinbin Chen

4. Markers of Cellular Senescence

Amancio Carnero

5. Biomarkers of Cell Senescence Assessed by Imaging Cytometry

Hong Zhao and Zbigniew Darzynkiewicz

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