Chloroplast Biotechnology Methods and Protocols [Paperback]

In Chloroplast Biotechnology: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used in chloroplast molecular biology.?Chapters focus on essential background information, applications in tobacco and protocols for plastid transformation in crops and Chlamydomonas and Bryophytes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoidance of known pitfalls.

Authoritative and practical, ?Chloroplast Biotechnology: Methods and Protocols seek to aid scientists who study chloroplast molecular biology as well as those interested in applications in agriculture, industrial biotechnology and healthcare.

Part I:? Background

1. The Plastid Genomes of Flowering Plants

Tracey A. Ruhlman and Robert K. Jansen

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2. Next Generation Technologies to Determine Plastid Genome Sequences

Robert J. Henry, Nicole Rice, Mark Edwards and Catherine J. Nock

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3. Plastid Gene Transcription: Promoters and RNA Polymerases

Jennifer Ortelt and Gerhard Link

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4. Plastid mRNA Translation

Masahiro Sugiura

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5. Engineering Chloroplasts for High-Level Foreign Protein Expression

Ralph Bock

6. Excision of Plastid Marker Genes Using Directly Repeated DNA Sequences

Elisabeth A. Mudd, Panagiotis Madesis, Elena Martin Avila and Anil Day

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7. Fluorescent Labeling and Confocal Microscopic Imaging of Chloroplasts and lÁ