Cholesterol Homeostasis Methods and Protocols [Hardcover]

This volume provides state-of-the-art techniques for studying various aspects of cholesterol homeostasis, including its uptake, synthesis and efflux from the cell, as well as its trafficking within the cell.? Chapters also cover techniques for studying the regulation of cholesterol homeostasis at both the transcriptional and post-translational levels, as well as studying the membrane topology and structure of cholesterol-related proteins.?Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cholesterol Homeostasis: Methods and Protocols?aims to provide key techniques in tackling the investigation of cholesterol homeostasis.

1. An Overview of Cholesterol Homeostasis

Ingrid C. Gelissen and Andrew J. Brown

2. Hybrid In Silico/In Vitro Approaches for the Identification of Functional Cholesterol Binding Domains in Membrane Proteins

Coralie Di Scala and Jacques Fantini

3. Structural Stringency of Cholesterol for Membrane Protein Function utilizing Stereoisomers as Novel Tools: A Review?

Md. Jafurulla and Amitabha Chattopadhyay

4. Manipulating Cholesterol Status within Cells

Winnie Luu, Ingrid C. Gelissen, and Andrew J. Brown

Anke Loregger, Jessica Kristine Nelson, and Noam Zelcer

6. The use of L-sIDOL Transgenic Mice as a Murine Model to Study Hypercholesterolaemia and Atherosclerosis

Eser J. Zerenturk and Anna C. Calkin