Dopamine Receptor Technologies [Paperback]

To foster a better understanding of dopamine receptor functionality, this detailed volume creates an interface between updated classical methods and new emerging technologies heretofore not available to new or seasoned researchers. Divided in five sections dedicated to experimental approaches investigating different facets of dopaminergic signal transduction, Dopamine Receptor Technologies covers epigenetic and post-transcriptional analysis, computational and biochemical techniques, visualization and imaging methods, molecular and cell biological tools, as well as behavioral assessment. The book, as a part of the popular Neuromethods series, provides insightful step-by-step protocols and methodological reviews that readers will find useful.

Practical and versatile, Dopamine Receptor Technologies seeks to aid researchers in developing new pharmacological tools to improve our knowledge of in vivo roles played by each receptor subtype and the synthesis of prospective lead compounds for drug discovery.

Part I: Genetic, Epigenetic, and Post-Transcriptional Analysis of Dopamine Receptors

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1. Genetic and Epigenetic Methods for Analysis of the Dopamine D2 Receptor Gene

??????????? Laura M. Fiori, Mireille Daigle, and Paul R. Albert

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2. Characterization of D1 Dopamine Receptor Post-Transcriptional Regulation

??????????? Eldo V. Kuzhikandathil

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Part II: Computational and Biochemical Methods in the Investigation of Dopamine Receptor Structure, Binding, and Post-Translational Regulation

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3. Computational Approaches in the Structure-Function Studies of Dopamine Receptors

??????????? Mayako Michino and Lei Shi

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4. Cell-Free Protein Synthesis andlƒG