Drosophila Cytogenetics Protocols [Hardcover]

Leading drosophilists describe in step-by-step detail all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. The chapters emphasize specimen preparation (from dissection to mounting) and cover both polytene and mitotic/meiotic chromosomes in depth. Each fully tested and readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. A cutting-edge FISH and immunolocalization technique will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression.Leading drosophilists describe in step-by-step detail all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. The chapters emphasize specimen preparation (from dissection to mounting) and cover both polytene and mitotic/meiotic chromosomes in depth. Each fully tested and readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. A cutting-edge FISH and immunolocalization technique will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression.The Chromosomes of Drosophila melanogasterDaryl S. HendersonSpermatogenesis: Analysis of Meiosis and MorphogenesisHelen White-CooperTime-Lapse Imaging of Male Meiosis by Phase-Contrast and Fluorescence MicroscopyElena Rebollo and Cayetano Gonz?lezImmunocytological Analysis of OogenesisEndre M?th?Cytological Analysis of OogenesisSeppo Nokkala and Christina NokkalaPolytene Chromosomes From Ovarian Nurse Cells of Drosophila melanogaster otu MutantsDmitry E. Koryakov, Natalia I. Mal'ceva, Robert C. King, and Igor F. ZhimulevFeulgen-DNA Cytophotometry for Estimating C ValuesEllen M. RaschFluorescent BrdU Labeling and Nuclear Flow Sorting of the Drosophila OvaryBrian R. Calvi alcÅ