ELISA Theory and Practice [Paperback]

Basic Immunology:1. The Immune Response2. Antigens3. Adaptive Immunity and Clonal Selection4. Antibodies5. Antigenic CommonnessBasic Principles of ELISA:1. Reaction Schemes2. Direct ELISA3. Indirect ELISA4. Sandwich ELISA5. Competition ELISA6. Choice of AssaysStages in ELISA:1. Solid-Phase2. Washing3. Addition of Reagents4. Incubation5. Blocking Conditions and Nonspecific Reactions6. Enzyme Conjugates7. Availability of Conjugates8. Conjugation with Enzymes9. Development of Label10. Stopping Reactions11. Reading12. Practical ProblemsTheoretical Considerations:1. Setting Up and Use of ELISA2. What Is Already Known3. Complexity of Problems4. Antigenic Considerations5. Other Techniques6. Units7. Making Dilutions8. Pipets9. MolaritiesDirect ELISA:Titration of Antigen and Antibody:1. Learning Principles2. Reaction Scheme3. Basis of Assay4. Materials and Equipment5. Practical Details6. Data Explained7. Aspects of Assay Described8. ConclusionsIndirect ELISA:1. Learning Principles2. Basis of Assay3. Materials and Reagents4. Practical5. Use of Indirect ELISA to Titrate Antibodies6. Use of Indirect ELISA to Determine the Positivity of Sera at Single DilutionUse of Antibodies on Solid-Phase in Capture ELISA:1. Use of Capture ELISA to Detect and Titrate Antigen2. Reaction Scheme3. Basis of Assay4. Materials5. Methods6. Titration of Capture Antibody Using IgG7. Titration of Capture Antibody when Used as Whole Serum8. Use of Capture ELISA to Detect Antigens9. Use of Capture ELISA to Detect and Titrate AntibodiesCompetitive ELISA:1. General Information2. Direct Competitive ELISA for Antigen Detection and Quantification3. Indirect Assay-Antigen Competition4. Indirect Competition Assay for Antibody Detection5. Indirect Assay Competition for Antibody-Detection Using a Single Dilution of Test Serum6. Overall Conclusions on Competition AssaysImmunochemical Techniques:1. Introduction2. Labeling Antibodies with Enzymes3. Coupling Antibodies to Alkaline Phosphatase4. Preparation of ImmunoglsH