Bringing together techniques and methods currently being applied to the study of exocytosis, Exocytosis Methods collects chapters from experts in the field, examining this fundamental process essential to functions ranging from protein secretion to hormone release and neurotransmission.? The book begins with a section covering a range of techniques being applied to the study of single-vesicle fusion events, which are key in order to gain insight into the final steps of vesicle fusion.? The volume continues with several model systems that are being employed to unravel the complexities of exocytosis.? Written for the Neuromethods series, chapters included in this work present the kind of detail and vital implementation advice that leads to successful results.
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Practical and authoritative, Exocytosis Methods seeks to promote the advent of new methods in microscopy and the development of new preparations, which would doubtlessly lead to many new and exciting discoveries in this field.
This book features cutting-edge techniques and methods for studying exocytosis in the brain. It provides expert implementation advice and step-by-step detail essential for reproducible results.
Part I: Techniques in the Measurement of Exocytosis
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1. Solution Single-Vesicle Fusion Assay by Single-Molecule Alternating Laser Excitation
??????????? Jae-Yeol Kim, Bong-Kyu Choi, Yeon-Kyun Shin, and Nam Ki Lee
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2. Imaging the Stages of Exocytosis in Epithelial Type II Pneumocytes
??????????? Thomas Haller and Paul Dietl
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3. Carbon-Fibre Amperometry in the Study of Exocytosis
??????????? Michael D. Duffield, Ravinarayan Raghupathi, and Damien J. Keating
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4. Imaging of Insulin Exocyl#£
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