Genetics and Biotechnology of Lactic Acid Bacteria [Hardcover]

A prime reference volume for geneticists, food technologists and biotechnologists in the academic and industrial sectors. Fermentations with lactic acid bacteria determine important qualities such as taste, shelf-life, and food values. New methods of food production require fast and reliable manufacture, which has led to a dramatic surge of interest in the genetic, microbiological and biochemical properties of lactic acid bacteria.A prime reference volume for geneticists, food technologists and biotechnologists in the academic and industrial sectors. Fermentations with lactic acid bacteria determine important qualities such as taste, shelf-life, and food values. New methods of food production require fast and reliable manufacture, which has led to a dramatic surge of interest in the genetic, microbiological and biochemical properties of lactic acid bacteria.1 Gene transfer systems and transposition.- 1.1 Introduction.- 1.2 Conjugation.- 1.2.1 Heterologous conjugation systems.- 1.2.2 Homologous conjugation systems.- 1.3 Transduction.- 1.3.1 Transduction in Lactococcus spp..- 1.3.2 Transduction in Streptococcus thermophilus.- 1.3.3 Transduction in Lactobacillus spp..- 1.4 Transformation.- 1.4.1 Transformation systems.- 1.4.2 Protoplast transformation.- 1.4.3 Electrotransformation.- 1.4.4 Transfection.- 1.5 Protoplast fusion.- 1.6 Transposition.- 1.6.1 Insertion sequences.- 1.6.2 Heterologous transposons.- 1.6.3 Nisin transposons.- 1.7 Generalized recombination.- 1.7.1 Integration processes.- 1.7.2 Recombination genes.- 1.8 Chromosome mapping.- 1.9 Concluding remarks.- References.- 2 Gene cloning and expression systems in Lactococci.- 2.1 Introduction.- 2.2 Replicative gene cloning.- 2.2.1 Streptoccal plasmid vectors.- 2.2.2 Lactococcal plasmid vectors.- 2.2.3 Plasmid replication and stability.- 2.3 Integrative gene cloning.- 2.3.1 Integration vectors.- 2.3.2 Chromosomal gene amplification and stability.- 2.3.3 Integrative gene expression.- 2.4 Gene exprelã>