Guanylate Cyclase and Cyclic GMP Methods and Protocols [Hardcover]

Recently, many ground-breaking steps have been made towards ?better understanding NO/cGMP/PKG pathways, its components, substrates, and their localization within a given cell. These advances were only possible due to the development of sophisticated new techniques in the field. In Guanylate Cyclase and Cyclic GMP: Methods and Protocols expert researchers in the field seek to provide an overview of novel techniques to identify various elements of the NO/cGMP/PKG pathway and further characterize their function, signaling, localization, and importance on a cellular level and in whole animal models providing a higher patho-/physiological integration and relevance. ?Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

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Authoritative and practical, Guanylate Cyclase and Cyclic GMP: Methods and Protocols seeks to provide? scientist current methods and a useful guide towards the possibility to apply these techniques to their own research.

This Methods in Molecular Biology book offers methods to identify elements of the NO/cGMP/PKG pathway and to characterise function, signaling, localisation and importance on a cellular level and in whole animal models. Includes trouble-shooting tips and more.

1.NO-cGMP: The Past, the Present and the Future

Michael Russwurm, Corina Russwurm, Doris Koesling, and Evanthia Mergia

2. cGMP Dependent Protein Kinases (cGK)Franz Hofmann and J?rg W. Wegener

3. Enzyme Assays for cGMP Hydrolysing Phosphodiesterases

Sergei D. Rybalkin, Thomas R. Hinds and ?Joseph A. Blã&