Hox Genes Methods and Protocols [Paperback]

Hox Genes: Methods and Protocols explores techniques and methodologies which arose from or were successfully applied to the study of Hox genes and Hox proteins, at the intersection of experimental embryology, genetics, biochemistry, physiology, evolutionary biology and other life sciences. This detailed volume begins with a section on discovery and functional analysis of Hox genes and then it continues onward to discuss mode of action and biomedical applications of Hox proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Expert and practical, Hox Genes: Methods and Protocols serves as an ideal guide to researchers striving to move forward in this dynamic and exciting area of study.

Part I: Hox Genes: From Discovery to Functional Analysis

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1. Discovery and Classification of Homeobox Genes in Animal Genomes

??????????? Ferdinand Marl?taz, Jordi Paps, Ignacio Maeso, and Peter W.H. Holland

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2. How to Study Hox Gene Expression and Function in Mammalian Oocytes and Early Embryos

??????????? Delphine Paul, Caroline Sauvegarde, Ren? Rezsohazy, and Isabelle Donnay

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3. Genetic Lineage Tracing Analysis of Anterior Hox Expressing Cells

??????????? Brigitte Laforest, Nicolas Bertrand, and St?phane Zaffran

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4. A Genetic Strategy to Obtain P-Gal4 Elements in the Drosophila Hox Genes

??????????? Luis de Navas, David Foronda, Delia del Saz, and Ernesto S?nchez-Herrero

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