Human Fertility: Methods and Protocols is intended for all practitioners of reproductive medicine and ART, as well as for embryologists and reproductive, developmental, cell and molecular biologists and others in the biomedical sciences. The volume presents straight-forward manner best practice approaches for overcoming a host of fertility challenges. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Human Fertility: Methods and Protocols aids scientists in continuing to study assisted reproductive technologies.
Part I: Overview
1. General Aspects of Fertility and Infertility
Mark A. Damario
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2. Genetics of Male Fertility
Yi-Nan Lin and Martin M. Matzuk
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3. Genetics of Female Infertility due to Anomalies of the Ovary and Mullerian Ducts
Joe Leigh Simpson
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4. Gene Polymorphisms in Female Reproduction
Livio Casarini, Manuela Simoni
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5. Understanding the Spermatozoon
Queenie V. Neri, Jennifer Hu, Zev Rosenwaks, and Gianpiero D. Palermo
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6. Derivation of Human Embryonic Stem Cells (hESC)
Nikica Zaninovic, Qiansheng Zhan, and Zev Rosenwaks
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7. The Endocrinology of the Menstrual Cycle
Robert L. Barbieri
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8. Assisted Reproductive Techniques
Jack Yu Jen Huang and Zev Rlsˇ