In Situ Hybridisation Application to Developmental Biology and Medicine [Paperback]

This book brings together contributions from leaders in the application of in situ hybridisation.This book brings together contributions from leaders in the application of in situ hybridisation and guides the would-be exponent through the various options and variations of the technique. The information gained from this technique prompted advances in our understanding of developmental biology.This book brings together contributions from leaders in the application of in situ hybridisation and guides the would-be exponent through the various options and variations of the technique. The information gained from this technique prompted advances in our understanding of developmental biology.Advances in our understanding of biological mechanisms have frequently been associated with the development of new techniques. In situ hybridization is a classic case of just such an advance. The technique effectively combines histochemistry with molecular biology and enables the rapid analysis of the distribution of RNA, or DNA, in the tissues. The information gained from this has caused something of a revolution in our understanding of developmental biology, since a fundamental aspect of development is the spatial and temporal expression of genes. In addition the technique has found application in the field of medicine, where it is giving new insights into the functioning of healthy tissues and the diagnosis and study of diseases. This book brings together contributions from leaders in the application of this technique and will help the reader review and be guided through the various options and variations of the technique.1. Non radio isotopic labels for in situ hybridisation histochemistry: a histochemist's view G. Coulton; 2. Use of haptenised nucleic acid probes in fluorescent in situ hybridisation A. K. Raap; 3. The use of complementary RNA probes for the identification and localisation of peptide messenger RNA in the diffuse neuroendocrine system A. Giaid; 4. Contributions of thlSI