Innate DNA and RNA Recognition Methods and Protocols [Paperback]

Innate DNA and RNA Recognition: Method and Protocols presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Innate DNA and RNA Recognition: Method and Protocols provides a resource for immunologists, molecular biologists, virologists, microbiologists and researchers studying how the innate immune system handles nucleic acids from endogenous or foreign sources.

Part I: Analysis of Viral Nucleic Acid Sensing In-Silico and In-Vitro

1. Detection of RNA Modifications by HPLC Analysis and Competitive ELISA

Gernot Nees, Andreas Kaufmann and Stefan Bauer

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2. Enzymatic Synthesis and Purification of a Defined RIG-I Ligand

Marion Goldeck, Martin Schlee, Gunther Hartmann, and Veit Hornung

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3. Crystallization of Mouse RIG-I ATPase Domain: In situ Proteolysis

Filiz Civril and Karl-Peter Hopfner

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4. Isolation of RIG-I-associated RNAs from Virus Infected Cells

Andreas Schmidt, Andreas Linder, Nicolas Linder, and Simon Rothenfu?er

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5. Structure Modeling of Toll-like Receptors

Jing Gong and Tiandi Wei

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6. Nucleic Acid Recognition in Dendritic Cells

Alexander Heiseke, Katharina Eisen?cher, and Anne Krug

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7. Viral Nucleic Acid Recognition in Human Non-il³&