Macrophages A Practical Approach [Paperback]

Macrophages are an important part of the immune response and are characterized by their ability to phagocytose foreign matter. However the difficulties involved in macrophage isolation mean they are some of the body's least explored cells.Macrophage Methodologydescribes how to isolate moderate to high yields of viable cells from a variety of specific tissue sites under both normal and pathological conditions and then goes on to give protocols for macrophage purification. The third chapter covers techniques used to identify and measure endocytic and phagocytic capabilities using immunochemistry and fluorescent analysis. Chapter four identifies the key issues relating to the study of macrophages as antigen presenting cells and has protocols for the major assays used to measure antigen processing and presentation. Also covered are the theoretical and practical issues related to the processing and presentation of intracellular pathogens for which macrophages are the major host cell. The methods described for measuring macrophage secretory products concentrate on bioassays for molecules where no ELISA is available. The next two chapters cover measuring macrophage activityin vitroandin vivo. Finally methods are described for the analysis of gene expression in macrophages. A variety of broad techniques have been brought together in one affordable volume to makeMacrophage Methodologyan essential buy for anyone studying macrophages.

Isolation of macrophages from tissues, fluids, and immune response sites
Purification of macrophages
Characterization of macrophage antigens and receptors by immunochemistry and fluorescent analysis
Analysis of antigen processing and presentation
Macrophage secretory products
Analysis of macrophage lytic function
Analysis of macrophage activity in vivo
Analysis of gene expression in mononuclear phagocytes