Membrane Proteins - Production and Functional Characterization [Hardcover]

Membrane Proteins - Production and Function Characterization a volume of Methods in Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines.

• Volume of Methods In Enzymology
• Contains a collection of a diverse array of topics in the area of membrane protein biology ranging from recombinant expression, isolation, functional characterization, biophysical studies and crystallization

Section I: Recombinant Expression of Membrane Proteins
1.?Engineering Escherichia Coli for Functional Expression of Membrane Proteins
Franz Y Ho and Bert Poolman
2.?ACEMBLing a Multiprotein Transmembrane Complex:? The Functional SecYEG-SecDFYajC-YidC? Holotranslocon Protein Secretase/Insertase
Joanna Komar, Mathieu Botte, Ian Collinson, Christiane Schaffitzel and Imre Berger
3. ?Expression and Purification of OsVDAC4
Ashwini Godbole, Ashvini K. Dubey, Rohan Mitra, Palakolanu S. Reddy and M. K. Mathew
4.?Membrane Protein Expression in Lactococcus Lactis
Martin S. King, Christoph Boes and Edmund R.S. Kunji
5.?An Unconventional Anaerobic Membrane Protein Production System Based on Wolinella Succinogenes
Michael Lafontaine and C. Roy D. Lancaster
6.?Membrane Protein Expression and Analysis in Yeast
Katrien Claes, Mouna Guerfal and Nico Callewaert
7.?Heterologous Expression of G Protein Coupled Receptors in Yeast
Nicolas Bertheleme, Shweta Singh, Simon Dowell and Bernadette Byrne
8.?Recombinant G protein-Coupled Receptorl#"