Microarrays in Clinical Diagnostics [Paperback]

Leading academic and industrial investigators surveys the world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification, and quantification of proteins, and for studies of protein-protein interactions. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.Leading academic and industrial investigators surveys the world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification, and quantification of proteins, and for studies of protein-protein interactions. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.Noncontact Laser Microdissection and Pressure Catapulting: Sample Preparation for Genomic, Transcriptomic, and Proteomic AnalysisYilmaz Niyaz, Monika Stich, Bernd S?gm?ller, Renate Burgemeister, Gabriele Friedemann, Ulrich Sauer, Rainer Gangnus, and Karin Sch?tzeApplications of the Universal DNA Microarray in Molecular MedicineReyna Favis, Norman P. Gerry, Yu-Wei Cheng, and Francis BaranySensitive Detection of SARS Coronavirus RNA by a Novel Asymmetric Multiplex Nested RT-PCR Amplification Coupled With Oligonucleotide Microarray HybridizationZhi-wei Zhang, Yi-ming Zhou, Yanl3<