Neurochemistry A Practical Approach [Paperback]

Neurobiology has undergone a revolution with many advances dependent on new techniques and approaches. This fully updated second edition reflects the recent growth areas in this subject. Its major themes are the fractionation and study of cellular and subcellular components of the nervous system, chemical neuroanatomy, and the measurement of neuronal metabolismin vivoandin vitro. Topics include the neuronal cytoskeleton; isolation of synaptosomes and their subcellular components; aspects of mitochondrial defects in neurones; and synaptic transport processes exemplified by the GABA transporter. This is an invaluable practical laboratory manual for all researchers in neurobiology; it contains step-by-step protocols and a troubleshooting guide, as well as examples of experimental data and their interpretation.

1. Isolation of synaptosomes, growth cones and their subcellular components
2. Proteins of the neuronal cytoplasmic and membrane-associated cytoskeleton
3. Immunocytochemical techniques for the study of the nervous system
4. In situ hybridization approaches to the study of the nervous system
5. Investigation of mitochondrial defects in brain and skeletal muscle
6. Measurement of neurotransmitters and their associated second messengersin vivo
7. Protein phosphorylation and dephosphorylation in the nervous system
8. Receptor-linked phosphoinositide metabolism
9. Neurotransmitter transporters: purification, reconstitution, and assay of the brain GABA transporter