Nuclear Bodies and Noncoding RNAs Methods and Protocols [Hardcover]

This volume focuses on cytological, biochemical, and molecular biological methods to identify and examine the function of each nuclear body, with an emphasis on the analysis of long non-coding RNAs. Chapters focus on exploring recent studies that reveal how certain long non protein-coding RNAs accumulate in specific nuclear bodies and regulate the function of the bodies by serving as architectural components or controlling the dynamics of associating protein components. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Nuclear Bodies and Noncoding RNAs: Methods and Protocols serves as a guideline for further study into the sophisticated regulation of gene expression.

Part I: Imaging of Noncoding RNAs and Nuclear Bodies

1. Visualization of lncRNA by Single Molecule Fluorescence in situ Hybridization

????? Margaret Dunagin, Moran N. Cabili, John Rinn, and Arjun Raj

2.????? Super-resolution Imaging of Nuclear Bodies by STED Microscopy

Yasushi Okada and Shinichi Nakagawa

3.????? High-resolution 3D DNA FISH Using Plasmid Probes and Computational Correction of Optical Aberrations to Study Chromatin Structure at the Sub-megabase Scale

Luca Giorgetti, Tristan Piolot, and Edith Heard

4.????? Time-lapse Imaging of Nuclear Bodies

????? Saskia Hutten, Samuel Swift, and Angus I. Lamond

5.????? Visualization of Nucleic Acids with Synthetic Exciton-controlled Fluorescent Oligonucleotide Probes

Dan Ohtan Wang and Akimitsl£,