Photoswitching Proteins Methods and Protocols [Hardcover]

Photoswitching Proteins: Methods and Protocols, focuses on proven applications of photoswitching proteins in cell and neurobiology. Optical approaches have been mostly embraced by the neurosciences to allow fast, high-resolution characterization and manipulation of single cells in complex neuronal tissue. These tools, including photoswitching of proteins, are now successfully used in almost all fields of biological research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Photoswitching Proteins: Methods and Protocols?highlights many of the possibilities for research based on light-inducible, high-resolution manipulation of biological systems.

1. Photoinduced Damage Resulting from Fluorescence Imaging of Live Cells

Matthew K. Daddysman, Michael A. Tycon, and Christopher J. Fecko

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2. Modification of Purified Proteins with Photochemical Protection Compounds for High-Resolution Photoactivation of Protein Function In Vitro and In Vivo

Sidney B. Cambridge

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3. Optochemical Activation of Kinase Function in Live Cells

Andrei V. Karginov, Klaus M. Hahn, and Alexander Deiters

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4. Photoswitching of Cell Surface Receptors Using Tethered Ligands

Andreas Reiner and Ehud Y. Isacoff

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5. Photocontrol of AMPA Receptors with a Photochromic Ligand

Martin Sumser and Philipp Stawski

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6. Photoconversion of CFP to Sl4