Plant Cell Culture Essential Methods [Hardcover]

The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf.

• Highlights the best and most up-to-date techniques for working on plant cell culture
• Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches
• Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture

An essential laboratory manual for students and early-career researchers.

Preface.

Contributors.

1 Plant Micropropagation (Ivan Iliev, Alena Gajdosova, Gabriela Libiakova, Shri Mohan Jain).

1.1 Introduction.

1.2 Methods and approaches.

1.2.1 Explants and their surface disinfection.

1.2.2 Culture melC×