Plant Embryogenesis [Paperback]

• Step-by-step reproducible laboratory procedures written by the top experts in the field offering their time-tested hints, tricks, and tips.
• Presents detailed outlines of embryonic systems models along with cutting-edge cellular, genetic, and molecular mechanism.
• Provides the most comprehensive collection of protocols on plan embryo development.

The advent of the postgenome era has brought a remarkable evolution in modern plant embryology. In Plant Embryogenesis, the authors focus on the common developmental process and compile novel tools and methods designed specifically for studying plant embryogenesis. In each chapter, detailed outlines of model embryonic systems are provided, as well as cutting-edge cellular, genetic and molecular mechanisms. This important book gives insight into the methods used in studying the transformation of phylogenetically-conserved embryonic patterns into floras astounding diversity of growth forms today. Invaluable to those new to the field and inspiring to full-fledged plant embryologists, Plant Embryogenesis provides todays most comprehensive platform to the understanding of plant embryo development.

Contents PART I. MODEL EMBRYONIC SYSTEMS 1. Arabidopsis embryogenesis Soomin Park and John J. Harada 2. Maize embryogenesis Pilar Fontanet and Carlos M. Vicient 3. Spruce embryogenesis Sara von Arnold and David Clapham PART II. CELLULAR, GENETIC AND MOLECULAR MECHANISMS OF PLANT EMBRYOGENESIS 4. In vitro fertilization with isolated higher plant gametes Erhard Kranz, Yoichiro Hoshino and Takashi Okamoto 5. In vitro culture of Arabidopsis embryos Michael Sauer and Jir? Friml 6. Culture and time-lapse tracking of barley microspore-derived embryos Simone de F. Maraschin, Sandra van Bergen, Marco Vennik and Mei Wang 7. Isolation of embryo-specific mutants in Arl³ß