Plant Isoprenoids Methods and Protocols [Paperback]

Plant Isoprenoids: Methods and Protocols is a collection of detailed techniques that will be a useful tool for a wide range of plant biologists, as well as for scientists of other fields interested in plant isoprenoids. Isoprenoids are an incredibly diverse family and they participate in a large variety of processes. Divided into four convenient sections to better cover strategic areas in plant isoprenoid research, topics include measurement of core enzyme activities involved in the production of isoprenoid precursors, targeted analysis of major groups of isoprenoid metabolites, isoprenoid profiling in specialized organs such as trichomes and oil glands as well as genetic, pharmacological and bioinformatic tools that are particularly useful for plant molecular biologists. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Plant Isoprenoids: Methods and Protocols will serve as an excellent reference material that can be adapted to develop customized methods for different needs making the world of plant isoprenoids more accessible for all researchers.

1. Plant Isoprenoids: A General Overview

Manuel Rodr?guez-Concepci?n

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I. Measurement of Core Enzyme Activities

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2. Measuring the Activity of 1-Deoxy-D-Xylulose 5-Phosphate Synthase, the First Enzyme in the MEP Pathway, in Plant Extracts

Louwrance P. Wright and Michael A. Phillips

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3. Determination of 3-Hydroxy-3-methylglutaryl CoA Reductase Activity in Plants

Narciso Campos, Montserrat Arr?, Albertl#+