Plant Protoplasts and Genetic Engineering III [Paperback]

Section I Regeneration of Plants from Protoplasts.- I.1 Regeneration of Plants from Protoplasts of Agrostis alba (Redtop).- 1 Introduction.- 2 Induction of Embryogenic Callus and Establishment of Embryogenic Suspension Culture.- 3 Isolation of Protoplasts.- 4 Culture of Protoplasts.- 5 Regeneration of Plants.- 6 Conclusions.- 7 Protocol.- References.- I.2 Regeneration of Plants from Protoplasts of Atropa belladonna L. (Deadly Nightshade).- 1 Introduction.- 2 Isolation and Culture of Protoplasts.- 3 Protoplast Fusion and Somatic Hybridization.- 4 Cryopreservation of Protoplasts.- 5 Summary.- 6 Protocol.- References.- I.3 Regeneration of Plants from Protoplasts of Brassica alboglabra Bailey (Chinese Kale).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Regeneration and Acclimatization of Plants.- 5 Characterization of Protoplast-Derived Plants.- 6 Gene Transfer into Protoplasts Using Electroporation.- 7 Conclusions and Prospects.- 8 Protocol.- References.- I.4 Regeneration of Plants from Protoplasts of Cucumis sativus L. (Cucumber).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Regeneration of Plants.- 5 Fusion of Protoplasts.- 6 Conclusions and Prospects.- 7 Protocol for Isolation, Culture, and Regeneration of Cucumber Protoplasts.- References.- I.5 Regeneration of Plants from Protoplasts of Lolium (Ryegrasses) and Festuca (Fescues).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Culture Media and Conditions.- 5 Regeneration from Protoplast Colonies.- 6 Somaclonal Variation in Regenerated Plants.- 7 Summary and Conclusions.- 8 Protocol.- References.- I.6 Regeneration of Plants from Protoplasts of Lotus spp. (Birdsfoot trefoil).- 1 Introduction.- 2 Protoplast Isolation.- 3 Protoplast Culture and Plant Regeneration.- 4 Conclusion and Prospects.- 5 Protocol.- References.- I.7 Regeneration of Plants from Protoplasts of Lycium barbarum L. (Wolfberry).- 1 Introduction.- 2 Plant MateriallÃ9