RNA Scaffolds Methods and Protocols [Hardcover]

This volume provides techniques on recent developments that use RNA scaffolds as molecular tools. RNA Scaffolds: Methods and Protocols guides readers through methods on various domains as molecular biology, nanotechnologies, and structural biology. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, RNA Scaffolds: Methods and Protocols will serve as an invaluable reference for those interested in further study into this fascinating field.

1. A Method to Predict the 3D Structure of an RNA Scaffold

Xiaojun Xu and Shi-Jie Chen

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2. Post-crystallization Improvement of RNA Crystal Diffraction Quality

Jinwei Zhang and Adrian R. Ferr?-DAmar?

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3. Expression and Purification of RNA-protein Complexes in Escherichia coli?

El Khouri M, Catala M, Seijo B, Chabal J, Tisn? C, Dardel F, and ?Ponchon L

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4. Production of Homogeneous Recombinant RNA using a tRNA Scaffold and Hammerhead Ribozymes

Frank H.T. Nelissen, Hans A. Heus, and Sybren S. Wijmenga

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5. In vivo Production of Small Recombinant RNAs Embedded in 5S rRNA-Derived Protective Scaffold

Victor G. Stepanov and George E. Fox

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6. Detection of RNA-Protein Interactions using Tethered RNA Affinity Capture

IIOKA H. and Macara I.G.

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7. A Universal Method for Labeling Native RNA in Live Bacterial Cells

Irina Smolina and Natalia Broude

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