Serotonin Receptor Technologies [Paperback]

Reflecting a vast amount of new information concerning the functional characteristics of the various 5-HT receptor subtypes and the Na⁺-dependent serotonin transporter (SERT), this volume provides state of the art methodologies currently applied in serotonin research from leading experts in the field. Serotonin Receptor Technologies describes approaches that vary from molecular biological and biochemical techniques (e.g., regarding receptor dimerization), fluorescence microscopy and imaging applications, flow cytometry, the use of organotypic slice and cell cultures to the generation of genetically modified animal models and the development of sophisticated behavioral tests, thus covering a wide spectrum of techniques to study serotonergic signaling in detail. Written for the popular Neuromethods series, chapters include the kind of detail and practical advice that ensures successful results in the lab.

Authoritative and convenient, Serotonin Receptor Technologies serves to foster both basic and translational research aiming to further deepen our understanding of the various facets of aminergic systems, as well as to aid research on similar problems with related GPCRs and neurotransmitter transporters.

Part I: Receptor Dimerization

1. Novel Approaches to Serotonin Receptor Interaction StudiesSylwia Aukasiewicz, Ewa BBasiak, Kinga Szafran, and Marta Dziedzicka-Wasylewska

2. Techniques for the Study of GPCR Heteromerization in Living Cells and Animal Models, Jos? L. Moreno, Jeremy Seto, James B. Hanks, and Javier Gonz?lez-Maeso

Part II: Manipulation and Analysis of Serotonergic Signaling in Brain Slices

3. Organotypic Slices and Biolistic Transfection for the Study of Serotonin Receptor Function in CNS Neuron?Kelly McGregor, Jean-Claude Be?que, and Rodrigo Andrade