Site-Specific Recombinases Methods and Protocols [Hardcover]

This books aims to provide an up-to-date guide to using recombinases in the laboratory.? Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Site-Specific Recombinases: Methods and Protocols aims to serve as a valuable resource for working with recombinases, and inspires further creativity in the field.

Ivo J. Huijbers

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2. Direct Generation of Conditional Alleles using CRISPR/Cas9 in Mouse Zygotes

Colin Pritchard, Lona J. Kroese, and Ivo J. Huijbers

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3. Building Cre Knockin Rat Lines using CRISPR/Cas9

Yuanwu Ma, Lianfeng Zhang, and Xingxu Huang

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4. Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases

Eugenia Voziyanova, Rachelle P. Anderson, and Yuri Voziyanov

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5. Use of the DICE Dual Integrase Cassette Exchange System

Alfonso P. Farruggio, Mital S. Bhakta, and Michele P. Calos

Avinash Chekuru, Veronika Kuscha, Stefan HanlT