Steroid Receptors Methods and Protocols [Hardcover]

Steroid Receptors: Methods and Protocols presents a selection of techniques that have been recently applied to the analysis of steroid receptors, powerful tools for the advancement of our understanding of both the mechanisms regulating gene transcription and the rapid signaling responses of tissues to signals. Research in this area has generated a wealth of data allowing the elucidation of steroid receptor mechanisms and improving the treatment of many endocrine disorders, above all cancers. Chapters cover methods to analyze gene transcription, chromatin and proteomic modifications, extra-nuclear signaling regulation, development of cell and animal models, and preparation of new antibodies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Steroid Receptors: Methods and Protocols offers an updated view on a variety of modern methods that will hopefully contribute to improving our knowledge on the integration of steroid receptors in single or different functionally connected cellular contexts under a variety of physiological and pathological conditions, above all tumors.

1. Progesterone Receptor Interaction with Chromatin

Guillermo P. Vicent, A. Silvina Nacht, Cecilia Ballar?, Roser Zaurin, Daniel Soronellas, and Miguel Beato

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2. Mapping the Genomic Binding Sites of the Activated Retinoid X Receptor in Murine Bone Marrow-Derived Macrophages using Chromatin Immunoprecipitation Sequencing

Benc?? Daniel, Balint L. Balint, Zsuzsanna S. Nagy, Laszlo L. Nagy

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3. Analysis of Chromatin-Nuclear Receptor Intl£*