Steroid-Protein Interactions II [Paperback]

I. Steroid-Protein Interactions Revisited.- II. Serum Albumin.- A. The Steroid Binding Site of BSA and HSA.- 1. Three-Dimensional Structure.- 2. Possible Residues Involved.- B. Relation of Fatty Acid to Steroid Binding.- C. Steroid Binding to Oligomers of HSA.- D. Characteristics of Steroid Binding to Albumin.- 1. Effect of Calcium Ions.- 2. Influence of Protein Concentration.- 3. Steroid Binding to Albumin from Various Species.- 4. Binding Specificity of BSA.- E. Spin Label Studies.- III. ?1-Acid Glycoprotein (AAG, Orosomucoid).- A. Isolation of AAG.- 1. From Human Blood and Tissues.- 2. AAG from Other Species.- B. Studies on AAG Synthesis.- 1. Effect of Inflammation.- 2. Effects of Endocrines.- C. Chemical Properties.- 1. Peptide Moiety.- 2. Carbohydrate Moiety.- D. Molecular Properties.- 1. Variants.- 2. Thermostability.- E. Interaction with Steroids.- 1. Binding to AAG and Its Variants.- 2. Inhibition by Lipids.- 3. Chemical Modification of Amino Acid Residues.- 4. Spectral Studies.- a) Ultraviolet Absorption.- b) Fluorescence Quenching.- 5. Electron Spin Resonance.- IV. Some Recent Methodological Developments.- 1. Measurement of Unbound Steroid.- 2. Adsorption and Related Methods.- 3. Electrophoretic Methods.- 4. Equilibrium Dialysis, Scatchard Plots and Related Procedures.- V. Corticosteroid-Binding Globulin (CBG).- A. Biosynthesis.- 1. Rat.- 2. Guinea Pig.- 3. Chicken.- B. Hormonal and Other Influences on Blood CBG Level.- 1. Rat.- 2. Guinea Pig.- 3. Birds.- C. General Characteristics of CBG in Nonmammalian Vertebrates.- 1. Corticosteroid-Binding in Fish Serum.- 2. Corticosteroid-Binding in Amphibian Serum.- 3. Corticosteroid-Binding in Reptile Plasma.- 4. Corticosteroid-Binding in the Blood of Birds.- a) Chicken.- b) Duck.- c) Sparrow.- D. General Characteristics of CBG in Nonhuman Mammals.- 1. Marsupials.- 2. Mouse.- 3. Rat.- a) CBG in the Prenatal and Developing Postnatal Rat.- b) CBG in Inflammation and Other Conditions.- c) Characteristics of Purified Ral#¶