Structural Genomics General Applications [Hardcover]

The field of Structural Genomics has produced many technological advances that transform and accelerate structure solution and analysis. Structural Genomics: General Applications emphasizes the benefits to the wider structural research community. It also reflects the current trend in tackling the more ambitious challenges of studying macromolecular machineries and complexes. Divided into three convenient sections, topics include the cloning and production of proteins for structural studies, experimental methods, and computational methods and data analysis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Structural Genomics: General Applications aims primarily to channel spin-off technologies to the average structural biologist in a small or medium-sized laboratory.

Part I. Cloning, Expression and Protein Production

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1. DisMeta  a Meta Server for Construct Design and Optimization

??????????? Yuanpeng Janet Huang, Thomas B. Acton, and Gaetano T. Montelione?????

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2. Stable Expression Clones and Auto-Induction for Protein Production in E. Coli

??????????? F. William Studier

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3. High-Throughput Expression Screening and Purification of Recombinant Proteins in E. coli

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