Since the first edition of Vaccinia Virus and Poxvirology: Methods and Protocols was published, a number of important events related to poxvirology have occurred, such as FDA approval of a culture-based live smallpox vaccine and the vaccination of large numbers of U.S. military and relatively large numbers of U.S. civilians.?Novel anti-poxvirus therapeutics have been developed and have been used in emergency settings. The second edition of Vaccinia Virus and Poxvirology expands upon the previous edition with entirely new sets of protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
?Authoritative and practical, Vaccinia Virus and Poxvirology: Methods and Protocols, Second Edition seeks to aid scientists in continuing to study poxviruses using new tools and approaches.
The second edition of this Methods in Molecular Biology volume details important progress in poxvirology, and offers entirely new sets of protocols. Includes materials and reagents lists, step-by-step lab protocols, and tips on pitfalls and troubleshooting.
1. Working Safely with Vaccinia Virus: Laboratory Technique and Review of Published Cases of Accidental Laboratory Infections
Stuart N. Isaacs
?2. In-Fusion? Cloning with Vaccinia Virus DNA Polymerase
Chad R. Irwin, Andrew Farmer, David O. Willer, and David H. Evans
?3. Genetic Manipulation of Poxviruses using Bacterial Artificial Chromosome Recombineering
Matthew G. Cottingham
?4. Easy and Efficient Protocols for Working with RelSU
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