This detailed book explores methods to isolate, characterize, and investigate key lung innate immune cells. Beginning with an overview, the volume then continues with methods for creating in vitro and in vivo model systems to study inflammatory lung diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge,
Lung Innate Immunity and Inflammation: Methods and Protocols aims to be a guidebook and will be of value and interest to researchers investigating innate immunity and inflammation in the lung as well as other organs and tissues.
Part I: Lung Innate, Immunity, and Inflammation
1. 500 Million Alveoli from 30,000 Feet: A Brief Primer on Lung Anatomy
William McKleroy and Kenneth Lyn-Kew
2. Overview of Innate Lung Immunity and Inflammation
David W.H. Riches and Thomas R. Martin
Part II: Isolation and Characterization of Lung Innate Immune Cells
3. Isolation and Characterization of Mononuclear Phagocytes in the Mouse Lung and Lymph Nodes
Sophie L. Gibbings and Claudia V. Jakubzick
4. Isolation and Characterization of Mouse Neutrophils
Niki D.J. Ubags and Benjamin T. Suratt
5. Isolation and Characterization of Mouse Fibroblasts
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