This volume explores the various facets of planaria as a biomedical model system and discusses techniques used to study the fascinating biology of these animals. The chapters in this book are divided into two parts: Part One looks at the biodiversity of planarian species, the molecular orchestration of regeneration, ecology of planarians in their natural habitats and their history as lab models. Part Two talks about experimental protocols for studying planarians, ranging from the establishment of a planarian research colony, to RNA and DNA extraction techniques, all the way to single stem cell transplantations or metabolomics analysis. Written in the highly successful
Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.((
Comprehensive and cutting-edge,
Planarian Regeneration: Methods and Protocols is a valuable resource for both newcomers to the field and experts within established planarian laboratories.
Preface&
Table of Contents&
Contributing Authors&
1. Planarian Diversity and Phylogeny
Ronald Sluys and Marta Riutort
2. Stem Cells, Patterning, and Regeneration in Planarians-Self-Organization at the Organismal Scale
Jochen Rink
3. The Ecology of Freshwater Planarians
Miquel Vila-Farr? and Jochen C. Rink
4. Planarians and the History of Animal Regeneration: Paradigm Shifts and Key Concepts in Biology
Sarah A. Elliott and Alejandro S?nchez Alvaral30