RNA Vaccines Methods and Protocols [Paperback]

This volume provides an overview of the field and practical hints for vaccinologists in academia and industry. Chapters provide protocols on self-replicating RNA vectors, non-replication mRNA vectors, adjuvantation and delivery, and preclinical and clinical development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, RNA Vaccines: Methods and Protocols aims to increased collaboration on RNA vaccines between basic and applied scientists in academia, government, and industry to develop future solutions for todays challenges.

1.      Introduction to RNA Vaccines

Thomas Kramps and Knut Elbers

 

 

2.      Self-replicating RNA

Birke A. Tews and Gregor Meyers

 

3.      Self-replicating RNA Vaccine Delivery to Dendritic Cells

Thomas D?moulins, Pavlos C. Englezou, Panagiota Milona, Nicolas Ruggli, Nicola Tirelli, Chantal Pichon, C?dric Sapet, Thomas Ebensen, Carlos A. Guzm?n, and Kenneth C. McCullough

 

4.      Plant Expression of Trans-encapsidated Viral Nanoparticle Vaccines with Animal RNA Replicons

Yiyang Zhou, Alison A. McCormick, and Christopher M. Kearney

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