This book presents a methodological introduction on clathrin-mediated endocytosis. Chapters guide readers through a number of model systems, purifications, reconstitutions, analysis techniques and systemic alterations of membrane function, which has been pivotal in understanding the role of clathrin and is panoply of interactors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge,
Clathrin-Mediated Endoytsis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
1. Purification of Clathrin-coated Vesicles from Adult Rat Brain
Silvia Gioved?
2. Preparation of Synaptosomes from Mammalian Brain by Subcellular Fractionation and Gradient Centrifugation
Mirko Messa
3. Probing Endocytosis during the Cell Cycle with Minimal Experimental Perturbation
Ant?nio JM Santos and Emmanuel Boucrot
4. Assaying the Contribution of Membrane Tension to Clathrin-mediated Endocytosis
Steeve Boulant
5Identifying Small Molecule Inhibitors of the Clathrin Terminal Domain
Volker Haucke and Michael Krau?
6. Acute Manipulations of Clathrin-mediated Endocytosis at Presynaptic Nerve Terminals
Rylie B. Walsh, Ona E. Bloom, and Jennifer R. Morgan
7. Imagil£,
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