Rheumatoid Arthritis Methods and Protocols [Hardcover]

This volume provides a summary of the standard laboratory protocols and methodology commonly used in basic and translational studies in the field of rheumatoid arthritis (RA) treatment. Chapters detail including basic RA models, evaluation of disease activity and immunological status, systemic drug delivery, and new research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Part I: Animal Models

1. Collagen-induced Arthritis Models

Miyoshi Maya and Shuang Liu

 

2. Human Xenograft Model

Shuang Liu

 

3. Long-term Constant Subcutaneous Drug Administration

Shuang Liu and Maya Miyoshi

 

4. Clinical Scoring of Disease Activity in Animal Models

Maya Miyoshi and Shuang Liu

 

5. Histological Analysis of Arthritic Joints

 

6. Preparation of Joint Extracts

Shuang Liu and Erika Takemasa

 

7. Production of Immunizing Antigen Proteoliposome Using Cell-free Protein Synthesis System

Wei Zhou and Hiroyuki Takeda