Rho GTPases Methods and Protocols [Hardcover]

This detailed book expands upon the previous edition with a collection of methods for those performing experimental work on small GTPases of the Rho family. Split into four sections, the volume explores computational modeling and imaging procedures, biochemical methods related to post-translational modifications of Rho GTPases as well as some high throughput methods, functional assays that allow for monitoring the consequences of manipulating Rho GTPases in a variety of cell types and cell biology processes, and techniques specifically designed for studies in selected non-mammalian model organisms (zebrafish, social ameba, plants and algae). Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble shooting and avoiding known pitfalls. 

Part I: Computational Modeling and Imaging

 

1. Computational Modeling of the Dynamics of Spatiotemporal Rho GTPase Signaling: A Systematic Review

            Shabnam Khatibi, Karina Islas Rios, and Lan K. Nguyen

 

2. Uncovering Bistability in the Rac1/RhoA Signaling Network through Integrating Computational Modeling and Experimentation

            Alexl³E