This detailed book provides state-of-the-art protocols to facilitate the construction of synthetic protein switches for a variety of applications in biotechnology and basic research. Beginning with general strategies, the volume continues with coverage of peptide switches, fluorescent and bioluminescent sensors, ?-lactamase sensors, proteolytic sensors, optogenetic switches, as well as cellular signaling switches. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Synthetic Protein Switches: Methods and Protocols serves as a useful reference for life scientists from diverse research fields that range from traditional, discovery-centered disciplines, like cancer research, to newly emerging disciplines, such as synthetic biology.
Part I: General Strategies and Considerations
1. Synthetic Protein Switches: Theoretical and Experimental Considerations
Viktor Stein
2. Construction of Allosteric Protein Switches by Alternate Frame Folding and Intermolecular Fragment Exchange
Jeung-Hoi Ha and Stewart N. Loh
3. Construction of Protein Switches by Domain Insertion and Directed Evolution
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