A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis [Paperback]

1 An Introduction to Avidin-Biotin Technology and Options for Biotinylation.- 1.1 Introduction.- The Avidin-Biotin Interaction.- Avidin.- Streptavidin.- Biotin.- 1.2 Technical Procedures.- Determining Biotin and Biotin Incorporation Levels.- Biotinylation Reagents.- General Considerations for Biotinylation.- Amine-Reactive Biotinylation Reagents.- Water-Soluble NHS Esters of Biotin.- Water-Insoluble NHS Esters of Biotin.- General Considerations for the Use of NHS-Ester Biotinylation Reagents.- Biotinylating IgG with NHS-Ester Biotinylation Reagents.- Carbohydrate-Directed Biotinylation Reagents.- Considerations for the Use of Hydrazide Derivatives of Biotin.- Carboxyl-Reactive Biotinylation Reagents.- Considerations for the Use of EDC.- Sulfhydryl-Reactive Biotinylation Reagents.- Considerations for the Use of Sulfhydryl-Reactive Biotinylation Reagents.- Biotinylation of Surface Thiols with Biotin-HPDP.- Biotinylating Reduced IgG with lodoacetyl-LC-Biotin.- Biotinylating Reduced IgG with Biotin-BMCC.- Phenylazide Derivatives of Biotin.- Considerations for the Use of Photoactivatable Biotin.- Biotinylation with Photoactivatable Biotin.- 1.3 Troubleshooting.- Avidin-HABA Biotin Assay.- NHS-Ester Biotinylation Reactions.- Biotin-Hydrazide Biotinylation Reactions.- EDC/5-(Biotinamido)pentylamine Reactions.- lodoacetyl-LC-Biotin Reactions.- Biotin-BMCC Reactions.- Phenylazide Biotinylation Reactions.- Acknowledgments.- References.- 2 Synthesis of Photocleavable Biotinylated Ligands and Application for Affinity Chromatography.- Summary.- 2.1 Introduction.- 2.2 Technical Procedures.- Chemical Synthesis.- 2.3 Results and Discussion.- Chemical Synthesis.- Binding to anti-CCK antisera and CCK-B receptors.- Binding to streptavidin agarose and photoelution.- Affinity chromatography.- 2.4 Troubleshooting.- References.- 3 Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands.- Summary.- 3.1 Introduction.- 3.2 TechnilSU